Volume 1 ; Issue 1 ; in Month : Jan-Dec (2019) Article No : 104
Velikova T, Ivanova-Todorova E, Kancheva L, et al.

Abstract

We aimed to evaluate a multiparametric test system for diagnosing autoimmune liver diseases in a Bulgarian cohort of patients. Methods: We investigated serum samples of 67 consecutive patients: twenty with autoimmune liver diseases (autoimmune hepatitis (AIH), primary biliary cirrhosis (PBC), and primary sclerosing cholangitis (PSC), fourty-seven with liver cirrhosis or viral hepatitis, and 20 healthy persons for the presence of antibodies against AMA-M2, M2-3E(BPO), Sp100, PML, gp210, LKM-1, LC-1, SLA/LP and Ro-52 by line blot technique, and AMA, anti-ASMA, ANA, and LKM antibodies by Indirect Immunofluorescence technique (IIF). Results: Twelve out of thirteen AIH patients showed antibodies against at least one of the tested antibodies (antibodies against SLA/LP, LKM-1, and anti-Ro52 (line blot), whereas 80% of them were positive for ANA and/or ASMA (IIF). We detected six out of six positive samples in the PBC group: for AMA-M2, anti-M2-3E (BPO), anti-Sp100 and anti-gp210. The PSC patient, as well as viral hepatitis group, stayed seronegative. We found a moderate correlation (r=0.67) and 100% coincidence between the results for AMA-M2 and LKM-1 antibodies testing by line blot and IIF. Conclusion: The investigated line immunoblot represents a useful diagnostic tool for AIH and PBC contributing positively to gold standard methods such as IIF.

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